Part:BBa_K1475007

GFP controlled by lacI and plac

This composite part has GFP controlled by a lacI, p(lacI) expression system.
Please note that this part contains the lacI repressor without the LVA rapid degradation tag. The lacI+LVA bacis part can be found here: Part:BBa_C0012.

Usage and Biology

This part can be used to test the expression from the lacI, p(lacI) system.
The operation of lacI repressor is inhibited by allolactose and [http://openwetware.org/wiki/IPTG IPTG (Isopropylthiogalactoside)]. IPTG is preferred because it cannot be degraded by Escherichia coli and therefore remains at a constant concentration.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 2057
  

References

1. Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional regulators. Nature 403, 335-338 (2000). http://www.nature.com/nature/journal/v403/n6767/full/403335a0.html
UniProt accesstion for Escherichia coli lacI: http://www.uniprot.org/uniprot/P03023
Entrez gene accession for Escherichia coli lacI: http://www.ncbi.nlm.nih.gov/gene?cmd=Retrieve&dopt=Graphics&list_uids=945007
Protein database entry for Escherichia coli lacI: http://www.rcsb.org/pdb/explore/explore.do?pdbId=1CJG